Composite

Part:BBa_K957006

Designed by: Dylan Patrick Webster   Group: iGEM12_Cornell   (2012-09-29)


Constitutive expression of nah Operon with oriT

One of two positively regulated operons of the NAH7 plasmid from Pseudomonas putida which encode the enzymes for metabolism of naphthalene via salicylate. When used with a BioBrick generator responsive to salicylate via the NahR activator (e.g. BBa_K957002 and BBa_K957003), this operon can function as part of a whole-cell biosensor for naphthalene.

Also included in this BioBrick part is an oriT sequence. When cloned into an existing non-conjugative BioBrick vector, the resulting plasmid may be inserted into a host via conjugation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1910
    Illegal PstI site found at 5905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4165
    Illegal PstI site found at 1910
    Illegal PstI site found at 5905
    Illegal NotI site found at 8208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5258
    Illegal BamHI site found at 3243
    Illegal BamHI site found at 5945
    Illegal XhoI site found at 1354
    Illegal XhoI site found at 3742
    Illegal XhoI site found at 3894
    Illegal XhoI site found at 9396
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1910
    Illegal PstI site found at 5905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1910
    Illegal PstI site found at 5905
    Illegal NgoMIV site found at 1847
    Illegal NgoMIV site found at 3622
    Illegal NgoMIV site found at 5496
    Illegal NgoMIV site found at 6108
    Illegal NgoMIV site found at 9421
    Illegal NgoMIV site found at 9577
    Illegal NgoMIV site found at 9587
    Illegal AgeI site found at 685
    Illegal AgeI site found at 3616
    Illegal AgeI site found at 6324
    Illegal AgeI site found at 8319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2964
    Illegal BsaI site found at 3637
    Illegal BsaI site found at 4228
    Illegal BsaI site found at 5394
    Illegal BsaI.rc site found at 2798
    Illegal BsaI.rc site found at 5548
    Illegal BsaI.rc site found at 7833
    Illegal SapI site found at 9479
    Illegal SapI.rc site found at 3328
    Illegal SapI.rc site found at 3874
    Illegal SapI.rc site found at 7725


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